Difference between revisions of "Sandbox"

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* '''Description:''' protein acetylase for the control of AcsA activity <br/><br/>
+
* '''Description:''' acetyl-CoA synthetase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''acuA''
+
|''acsA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || Gcn5-related N-acetyltransferase
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|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA synthetase)
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || control of AcsA activity
+
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of acetate, fatty acids
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.028  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.547  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 630 bp, 210 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1716 bp, 572 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acsA]]'', ''[[acuB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tyrS]]'', ''[[acuA]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:acuA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:acsA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acuABC.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acsA.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10369]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10370]
  
 
=== Additional information===
 
=== Additional information===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' acetylates (and thereby inactivates) [[AcsA]] on Lys-549  [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]
+
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' Gcn5-relatedN-acetyltransferase  [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed]
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Domains:'''  
 
* '''Domains:'''  
  
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* '''Modification:'''
+
* '''Modification:''' acetylated on Lys-549 by [[AcuA]], this results in inactivation  [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed], deacetylated by [[SrtN]] and [[AcuC]] deacetylates (and thereby activates) [[AcsA]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29690]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29680]
  
−
* '''E.C. number:'''
+
* '''E.C. number:''' 6.2.1.1
  
 
=== Additional information===
 
=== Additional information===
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* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
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* '''Regulation:''' repressed by glucose (3.4-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]   
+
* '''Regulation:''' repressed by glucose (4.5-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]   
  
 
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
 
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  

Revision as of 18:14, 15 April 2009

  • Description: acetyl-CoA synthetase

Gene name acsA
Synonyms
Essential no
Product acetyl-CoA synthetase)
Function utilization of acetate, fatty acids
MW, pI 64 kDa, 5.547
Gene length, protein length 1716 bp, 572 aa
Immediate neighbours tyrS, acuA
Hier soll was neues rein
Genetic context
AcsA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number: 6.2.1.1

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by glucose (4.5-fold) (CcpA) PubMed
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Gardner JG, Escalante-Semerena JC (2008) Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl coenzyme a synthetase (AcsA) in Bacillus subtilis. J. Bacteriol. 190: 5132-5136. PubMed
  3. Gardner JG, Escalante-Semerena JC (2009) In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase. J. Bacteriol. 191: 1749-1755. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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