acsA

acsA
168

acetyl-CoA synthetase

Locus
BSU_29680
Molecular weight
64.72 kDa
Isoelectric point
5.55
Protein length
572 aaSequenceBlast
Gene length
1719 bpSequenceBlast
Function
utilization of acetate, fatty acids
Product
acetyl-CoA synthetase)
Essential
no
E.C.
6.2.1.1
Synonyms
acsA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0365 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,038,213  3,039,931
The protein
Catalyzed reaction/ biological activity
acetate + ATP + CoA --> acetyl-CoA + AMP + diphosphate (according to UniProt)
Protein family
ATP-dependent AMP-binding enzyme family (according to UniProt)
Structure
8RPK (PDB) (from Chloroflexota bacterium, 41.8% identity) PubMed
P39062 (AlphaFold)
Modification
acetylated on Lys-549 by AcuA, this results in inactivation  PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA  PubMed
Paralogous protein(s)
YtcI, (35,1%)
Additional information
AcsA is degraded in the forespore using MdfA as the adaptor protein PubMed
Expression and Regulation
Operons
Genes
acsA-ytzK
Description
PubMed
Regulation
repressed by glucose (4.5-fold) (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
CodY: repression, PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

acsA→ytzK

2025-03-10 22:26:41

Jstuelk

80

be092fde38a5f881d569ea0d403fca7a757675c2

47CDD5FDE201249D77E00A0FC5B0180E09236624

Genes
acsA-ytzK-tyrS
Description
PubMed
Regulation
repressed by glucose (4.5-fold) (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
CodY: repression, PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

acsA→tyrS

2025-03-16 16:10:30

Jstuelk

115

64406ef718e7a3f606c729a8589083a4b11a12ee

AB9A54148FBC27339BE8203CF80C6070E696984B

Biological materials
Mutant
GP1212 (acsA::kan), available in Jörg Stülke's lab
BKE29680 (acsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCCAAACATCCCCCTT,  downstream forward: _UP4_ACAATGGAGGATTAAAAGGC
BKK29680 (acsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCCAAACATCCCCCTT,  downstream forward: _UP4_ACAATGGAGGATTAAAAGGC
References
Reviews
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Original Publications
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6671E7D85E99D349679F0E9D825DC035D10FFD2E

Page visits: 6092

Time of last update: 2025-03-31 07:29:42

Author of last update: Jstuelk