Difference between revisions of "Sandbox"

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* '''Description:''' trehalose-specific phosphotransferase system, EIIBC component <br/><br/>
+
* '''Description:''' phospho-alpha-glucosidase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''treP''
+
|''treA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''treB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''treC ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || trehalose-specific phosphotransferase system, EIIBC component
+
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-alpha-glucosidase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || trehalose uptake and phosphorylation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || trehalose utilization
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 8.555  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 4.895  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1410 bp, 470 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1683 bp, 561 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfkQ]]'', ''[[treA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[treP]]'', ''[[treR]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/treP_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/treA_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/treP_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/treA_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:treP_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:treA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 42: Line 42:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trePAR.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11009]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11010]
  
 
=== Additional information===
 
=== Additional information===
Line 81: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07800]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07810]
  
* '''E.C. number:'''
+
* '''E.C. number:''' 3.2.1.93
  
 
=== Additional information===
 
=== Additional information===
Line 101: Line 101:
 
=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP1118 (spc), available in the [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
Line 120: Line 120:
  
 
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149  
 
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149  
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
# Helfert C, Gotsche S, Dahl MK. (1995) Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene. ''Mol Microbiol. '' '''Apr;16(1):''' 111-120. [http://www.ncbi.nlm.nih.gov/sites/entrez/7651129 PubMed]

Revision as of 00:48, 3 April 2009

  • Description: phospho-alpha-glucosidase

Gene name treA
Synonyms treC
Essential no
Product phospho-alpha-glucosidase
Function trehalose utilization
MW, pI 65 kDa, 4.895
Gene length, protein length 1683 bp, 561 aa
Immediate neighbours treP, treR
Gene sequence (+200bp) Protein sequence
Genetic context
TreA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number: 3.2.1.93

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant: GP1118 (spc), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149
  2. Helfert C, Gotsche S, Dahl MK. (1995) Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene. Mol Microbiol. Apr;16(1): 111-120. PubMed