IlvC

• Description: ketol-acid reductoisomerase (2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)
  
  

Gene name	ilvC
Synonyms
Essential	no
Product	ketol-acid reductoisomerase (2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)
Function	biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ilvC
Metabolic function and regulation of this protein in SubtiPathways: ilvC
MW, pI	37 kDa, 5.37
Gene length, protein length	1026 bp, 342 aa
Immediate neighbours	leuA, ilvH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

• Locus tag: BSU28290

Database entries

• BsubCyc: BSU28290

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH (according to Swiss-Prot)

• Protein family: ketol-acid reductoisomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-298 PubMed
  • phosphorylated on Ser-338 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28290

• Structure: 1NP3 (from Escherichia coli, 53% identity, 71% similarity) PubMed

• UniProt: P37253

• KEGG entry: [3]

• E.C. number: 1.1.1.86

Expression and regulation

• Operon: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD PubMed

• Expression browser: ilvC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
  • repressed by casamino acids PubMed
  • expression is stimulated in the presence of glucose PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY, T-box) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • CcpA: transcription activation PubMed
  • T-box: tRNA-controlled RNA switch that mediates termination/antitermination
  • TnrA: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 21254 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2878 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 75395 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 36887 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 50694 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP521 (in pAC5), available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody: