AckA

• Description: acetate kinase
  
  

Gene name	ackA
Synonyms
Essential	no
Product	acetate kinase
Function	overflow metabolism
Gene expression levels in SubtiExpress: ackA
Metabolic function and regulation of this protein in SubtiPathways: ackA
MW, pI	42 kDa, 5.191
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	moaB, ytxK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ATP synthesis, carbon core metabolism, most abundant proteins

This gene is a member of the following regulons

CcpA regulon, CodY regulon

The gene

Basic information

• Locus tag: BSU29470

Database entries

• BsubCyc: BSU29470

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)

• Protein family: acetokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU29470

• Structure: 2IIR (from Thermotoga maritima, 54% identity, 73% similarity)

• UniProt: P37877

• KEGG entry: [3]

• E.C. number: 2.7.2.1

Expression and regulation

• Operon: ackA PubMed

• Expression browser: ackA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (2.5 fold) (CcpA) PubMed
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription activation PubMed
  • CcpA: transcription activation PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3042 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1758 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2419 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

Reviews

Cheryl Ingram-Smith, Stephen R Martin, Kerry S Smith
Acetate kinase: not just a bacterial enzyme.
Trends Microbiol: 2006, 14(6);249-53
[PubMed:16678422] [WorldCat.org] [DOI] (P p)

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)