RsbR

• Description: activator of RsbT kinase activity, stressosome sensor protein

Gene name	rsbR
Synonyms	ycxR, rsbRA
Essential	no
Product	activator of RsbT kinase activity, stressosome sensor protein
Function	control of SigB activity
Gene expression levels in SubtiExpress: rsbR
Interactions involving this protein in SubtInteract: RbsR
Metabolic function and regulation of this protein in SubtiPathways: rsbR
MW, pI	30 kDa, 4.731
Gene length, protein length	822 bp, 274 aa
Immediate neighbours	ndoA, rsbS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, phosphoproteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU04670

Database entries

• BsubCyc: BSU04670

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • positive regulator of YtvA-dependent light activation of the SigB stress response PubMed
  • phosphorylated RsbR activates the kinase activity of RsbT PubMed

• Protein family:

• Paralogous protein(s): RsbRB, RsbRC, RsbRD, YtvA

Extended information on the protein

• Kinetic information:

• Domains:
  • RsbRA is composed of an N-terminal nonheme globin domain and a highly conserved C-terminal STAS (Sulphate Transporter and AntiSigma factor antagonist) domain. The C-terminal STAS domain is the target of the serine/threonine-specific kinase RsbT (see below).

• Modification: phosphorylation on Thr-171 and Thr-205 by RsbT PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RsbX-RsbR PubMed
  • RsbR-RsbT PubMed
  • RsbR-RsbS
  • YtvA-RsbR PubMed

• • component of the stressosome

• Localization:

Database entries

• BsubCyc: BSU04670

• Structure: 2BNL (N-terminal domain), 3VY9 (complete stressosome)

• UniProt: P42409

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed

• Expression browser: rsbR PubMed

• Sigma factor: SigA PubMed

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage
• Rick Lewis, Newcastle, UK homepage