Difference between revisions of "MgsA"
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=== Additional information=== | === Additional information=== | ||
| − | |||
=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 125: | Line 124: | ||
* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP67 (''mgsA''::''tet''), available in [[Stülke]] lab | + | ** GP67 (''mgsA''::''tet'') {{PubMed|21992469}}, available in [[Jörg Stülke]]'s lab |
| − | ** GP84 (''mgsA''::''pX2''(''cat'')), available in [[Stülke]] lab | + | ** GP84 (''mgsA''::''pX2''(''cat'')), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Stülke]] lab | + | ** pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab |
| − | ** pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | + | ** pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Jörg Stülke]]'s lab |
| − | ** pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Stülke]] lab | + | ** pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Jörg Stülke]]'s lab |
| − | ** pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP1459]]), available in [[Stülke]] lab | + | ** pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP1459]]), available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| Line 138: | Line 137: | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab {{PubMed|21992469}} |
| − | * '''FLAG-tag construct:''' GP86 (spc, based on [[pGP1331]]), available in | + | * '''FLAG-tag construct:''' GP86 (spc, based on [[pGP1331]]) {{PubMed|21992469}}, available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
| − | [[Boris Görke]], University of Göttingen, Germany | + | * [[Boris Görke]], University of Vienna, Austria |
| − | [http:// | + | * [[JörgStülke]], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage] |
=Your additional remarks= | =Your additional remarks= | ||
Revision as of 17:15, 16 January 2014
- Description: methylglyoxal synthase
| Gene name | mgsA |
| Synonyms | ypjF, jojF |
| Essential | no |
| Product | methylglyoxal synthase |
| Function | bypass of glycolysis |
| Gene expression levels in SubtiExpress: mgsA | |
| Interactions involving this protein in SubtInteract: MgsA | |
| Metabolic function and regulation of this protein in SubtiPathways: mgsA | |
| MW, pI | 14 kDa, 4.919 |
| Gene length, protein length | 411 bp, 137 aa |
| Immediate neighbours | bshB1, dapB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22480
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
- Protein family: RNase Z family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- UniProt: P42980
- KEGG entry: [2]
- E.C. number: 4.2.3.3
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- GP67 (mgsA::tet) PubMed, available in Jörg Stülke's lab
- GP84 (mgsA::pX2(cat)), available in Jörg Stülke's lab
- Expression vector:
- pGP1301 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
- pGP1180 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
- pGP1181 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Jörg Stülke's lab
- pGP2207 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP1459), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab PubMed
- FLAG-tag construct: GP86 (spc, based on pGP1331) PubMed, available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
- Boris Görke, University of Vienna, Austria
- JörgStülke, University of Göttingen, Germany, Homepage
Your additional remarks
References
