mgsA

mgsA
168

methylglyoxal synthase

Locus
BSU_22480
Molecular weight
14.99 kDa
Isoelectric point
4.92
Protein length
137 aaSequenceBlast
Gene length
414 bpSequenceBlast
Function
bypass of glycolysis
Product
methylglyoxal synthase
Essential
no
E.C.
4.2.3.3
Synonyms
mgsA, ypjF, jojF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1803 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,358,911  2,359,324
The protein
Catalyzed reaction/ biological activity
dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)
Protein family
methylglyoxal synthase family (single member, according to UniProt)
Domains
MGS-like domain (aa 1-137) (according to UniProt)
Structure
6F2C (PDB) PubMed
P42980 (AlphaFold)
Effectors of protein activity
non-phosphorylated Crh interacts with MgsA to inhibit its activity PubMed
Expression and Regulation
Operons
Genes
ypjD-dapB-mgsA-bshB1-bshA-cca-birA
Description
PubMed
Regulation
induced by diamide stess (thiol depletion) (Spx) PubMed
Regulatory mechanism
Spx: activation, PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

ypjD→birA

2025-03-13 06:13:52

ghost

92

53bf67b167bd77fe013a5e22a2f6ddcbf0c547e2

73042872A92944881DD9D94DF293F1857E4735FB

Biological materials
Mutant
MGNA-A440 (mgsA::erm), available at the NBRP B. subtilis, Japan
GP67 (mgsA::tet) PubMed, available in Jörg Stülke's lab
GP84 (mgsA::pX2(cat)), available in Jörg Stülke's lab
BKE22480 (mgsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTTTCATTGTTTATCCCC,  downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
BKK22480 (mgsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTTTCATTGTTTATCCCC,  downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
Expression vectors
pGP1301 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP1180 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
pGP1181 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Jörg Stülke's lab
pGP2207 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP1459), available in Jörg Stülke's lab
pGP2505 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab PubMed
FLAG-tag construct
GP86 (spc, based on pGP1331) PubMed, available in Jörg Stülke's lab
Labs working on this gene/protein
Boris Görke, University of Vienna, Austria
Jörg Stülke, University of Göttingen, Germany, Homepage
References
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341F198B2BD4261B20C00181D084A7C5E33E39B3

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Time of last update: 2025-04-03 19:16:14

Author of last update: Jstuelk