AcdA

• Description: acyl-CoA dehydrogenase
  
  

Gene name	acdA
Synonyms
Essential	no
Product	acyl-CoA dehydrogenase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: acdA
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	41 kDa, 4.951
Gene length, protein length	1137 bp, 379 aa
Immediate neighbours	rpoE, fadF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

FadR regulon

The gene

Basic information

• Locus tag: BSU37170

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF (according to Swiss-Prot)

• Protein family: acyl-CoA dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YngJ, MmgC, FadE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2Z1Q (from Thermus thermophilus hb8, 40% identity, 55% similarity)

• UniProt: P45867

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fadF-acdA-rpoE PubMed

• Expression browser: acdA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of long-chain fatty acids (FadR) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)