Difference between revisions of "NrdF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates
 
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17390 nrdF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17390 nrdF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NrdF NrdF]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NrdF NrdF]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13623&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17390 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]]

Revision as of 12:54, 13 May 2013

  • Description: ribonucleoside-diphosphate reductase (major subunit)

Gene name nrdF
Synonyms
Essential yes PubMed
Product ribonucleoside-diphosphate reductase (major subunit)
Function synthesis of deoxyribonucleoside triphosphates
Gene expression levels in SubtiExpress: nrdF
Interactions involving this protein in SubtInteract: NrdF
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 38 kDa, 4.66
Gene length, protein length 987 bp, 329 aa
Immediate neighbours nrdE, ymaB
Sequences Protein DNA Advanced_DNA
Genetic context
NrdF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NrdF expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

This gene is a member of the following regulons

ResD regulon

The gene

Basic information

  • Locus tag: BSU17390

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
  • Protein family: ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
  • Paralogous protein(s): YosP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (ResD) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

Your additional remarks

References

Reviews

John Herrick, Bianca Sclavi
Ribonucleotide reductase and the regulation of DNA replication: an old story and an ancient heritage.
Mol Microbiol: 2007, 63(1);22-34
[PubMed:17229208] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed

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