Difference between revisions of "YflG"

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|style="background:#ABCDEF;" align="center"|'''Function''' || removal of N-terminal methionine from nascent proteins
 
|style="background:#ABCDEF;" align="center"|'''Function''' || removal of N-terminal methionine from nascent proteins
 
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|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07690 yflG]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07690 yflG]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.578   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.578   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflH]]'', ''[[nagP]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflH]]'', ''[[nagP]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12598&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07690 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yflG_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yflG_context.gif]]

Revision as of 12:21, 13 May 2013

  • Description: methionine aminopeptidase

Gene name yflG
Synonyms
Essential no
Product methionine aminopeptidase
Function removal of N-terminal methionine from nascent proteins
Gene expression levels in SubtiExpress: yflG
MW, pI 27 kDa, 5.578
Gene length, protein length 747 bp, 249 aa
Immediate neighbours yflH, nagP
Sequences Protein DNA Advanced_DNA
Genetic context
YflG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YflG expression.png
























Categories containing this gene/protein

translation, protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07690

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides (according to Swiss-Prot)
  • Protein family: peptidase M24A family (according to Swiss-Prot)
  • Paralogous protein(s): Map

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • NagR: transcription repression (weak and not certain) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

CongHui You, HongYan Lu, Agnieszka Sekowska, Gang Fang, YiPing Wang, Anne-Marie Gilles, Antoine Danchin
The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis.
BMC Microbiol: 2005, 5;57
[PubMed:16207374] [WorldCat.org] [DOI] (I e)