Difference between revisions of "YacF"
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* '''Operon:''' ''[[pabB]]-[[pabA]]-[[pabC]]-[[sul]]-[[folB]]-[[folK]]-[[yazB]]-[[yacF]]-[[lysS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9084182 PubMed] | * '''Operon:''' ''[[pabB]]-[[pabA]]-[[pabC]]-[[sul]]-[[folB]]-[[folK]]-[[yazB]]-[[yacF]]-[[lysS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9084182 PubMed] | ||
| − | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dusB_87634_88635_1 yacF] {{PubMed|22383849}} |
| + | |||
| + | * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9084182 PubMed] | ||
* '''Regulation:''' | * '''Regulation:''' | ||
Revision as of 14:33, 7 August 2012
- Description: putative tRNA-dihydrouridine synthase B
| Gene name | yacF |
| Synonyms | dusB, dus1 |
| Essential | no |
| Product | putative tRNA-dihydrouridine synthase B |
| Function | tRNA maturation |
| Metabolic function and regulation of this protein in SubtiPathways: Folate | |
| MW, pI | 36 kDa, 7.868 |
| Gene length, protein length | 999 bp, 333 aa |
| Immediate neighbours | yazB, lysS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00810
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: dus family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P37567
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
Antoine de Saizieu, Pierre Vankan, Cassandra Vockler, Adolphus P G M van Loon
The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon.
Microbiology (Reading): 1997, 143 ( Pt 3);979-989
[PubMed:9084182]
[WorldCat.org]
[DOI]
(P p)
