CcpC

• Description: transcriptional repressor of citZ and citB
  
  

Gene name	ccpC
Synonyms	ykuM
Essential	no
Product	transcriptional repressor (LysR family)
Function	regulation of tricarboxylic acid branch of the TCA cycle
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	33 kDa, 5.932
Gene length, protein length	879 bp, 293 aa
Immediate neighbours	ykuL, ykuN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

CcpA regulon, CcpC regulon

The CcpC regulon:

• ccpC, citB, citZ-icd-mdh

The gene

Basic information

• Locus tag: BSU14140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LysR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34827

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ykuJ-ykuK-abbA-ykuL-ccpC PubMed
  • ccpC PubMed

• Sigma factor:
  • ccpC: SigA

• Regulation:
  • repressed in the presence of glucose and glutamate (CcpC) PubMed

• Regulatory mechanism:
  • CcpC: transcription repression (molecular inducer: citrate) PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP206 (aphA3), available in Stülke lab

• Expression vector:
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP706, available in Stülke lab

• lacZ fusion: pGP707 (in pAC5), available in Stülke lab, pGP711 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage