Difference between revisions of "ComA"
(→Extended information on the protein) |
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| Line 81: | Line 81: | ||
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| − | * '''Effectors of protein activity:''' phosphorylation activates DNA binding | + | * '''Effectors of protein activity:''' |
| + | ** phosphorylation activates DNA binding | ||
| + | ** DNA-binding activity of ComA is inhibited by [[RapC]] {{PubMed|12950917}}, [[RapD]] {{PubMed|17227471}}, [[RapF]] {{PubMed|15968044,22215984}}, [[RapH]] {{PubMed|17581123}} and [[RapK]] {{PubMed|16816200}} | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
** [[RapC]]-[[ComA]] {{PubMed|12950917}} | ** [[RapC]]-[[ComA]] {{PubMed|12950917}} | ||
| − | ** [[RapF]]-[[ComA]] {{PubMed|15968044}} | + | ** [[RapF]]-[[ComA]] {{PubMed|15968044,22215984}} |
** [[RapH]]-[[ComA]] {{PubMed|17581123}} | ** [[RapH]]-[[ComA]] {{PubMed|17581123}} | ||
** [[ComP]]-[[ComA]] | ** [[ComP]]-[[ComA]] | ||
| Line 93: | Line 95: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1U83 1U83] | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=1U83 1U83] | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=3ULQ 3ULQ] (structure of the complex between the DNA-binding C-terminal domain of [[ComA]] with [[RapF]]) {{PubMed|22215984}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P14204 P14204] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P14204 P14204] | ||
| Line 138: | Line 142: | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>17227471 11133937,2116363,12067344,8387999,19202088, 10094672,8643670,11267663, 15968044 16816200, 10447896,12950917, 20302877 12270822 17581123 16816200 2507523 20208433 </pubmed> | + | <pubmed>17227471 11133937,2116363,12067344,8387999,19202088, 10094672,8643670,11267663, 15968044 16816200, 10447896,12950917, 20302877 12270822 17581123 16816200 2507523 20208433 22215984</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:01, 5 January 2012
- Description: two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)
| Gene name | comA |
| Synonyms | srfB, comAA |
| Essential | no |
| Product | two-component system response regulator |
| Function | regulation of genetic competence and quorum sensing |
| Interactions involving this protein in SubtInteract: ComA | |
| MW, pI | 23 kDa, 4.759 |
| Gene length, protein length | 642 bp, 214 aa |
| Immediate neighbours | yuxO, comP |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
genetic competence, transcription factors and their control, quorum sensing, phosphoproteins
This gene is a member of the following regulons
The ComA regulon
The gene
Basic information
- Locus tag: BSU31680
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: ComA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylated by ComP on an Asp residue
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P14204
- KEGG entry: [3]
- E.C. number: 4.4.1.19
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications
Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984]
[WorldCat.org]
[DOI]
(I p)
Carey A Hobbs, Benjamin G Bobay, Richele J Thompson, Marta Perego, John Cavanagh
NMR solution structure and DNA-binding model of the DNA-binding domain of competence protein A.
J Mol Biol: 2010, 398(2);248-63
[PubMed:20302877]
[WorldCat.org]
[DOI]
(I p)
Xiaoyu Wang, Chuping Luo, Youzhou Liu, Yafeng Nie, Yongfeng Liu, Rongsheng Zhang, Zhiyi Chen
Three non-aspartate amino acid mutations in the ComA Response regulator receiver motif severely decrease surfactin production, competence development and spore formation in Bacillus subtilis.
J Microbiol Biotechnol: 2010, 20(2);301-10
[PubMed:20208433]
[WorldCat.org]
(P p)
Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088]
[WorldCat.org]
[DOI]
(P p)
Wiep Klaas Smits, Cristina Bongiorni, Jan-Willem Veening, Leendert W Hamoen, Oscar P Kuipers, Marta Perego
Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);103-20
[PubMed:17581123]
[WorldCat.org]
[DOI]
(P p)
Mitsuo Ogura, Yasutaro Fujita
Bacillus subtilis rapD, a direct target of transcription repression by RghR, negatively regulates srfA expression.
FEMS Microbiol Lett: 2007, 268(1);73-80
[PubMed:17227471]
[WorldCat.org]
[DOI]
(P p)
Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200]
[WorldCat.org]
[DOI]
(P p)
Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044]
[WorldCat.org]
[DOI]
(P p)
Leighton Core, Marta Perego
TPR-mediated interaction of RapC with ComA inhibits response regulator-DNA binding for competence development in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1509-22
[PubMed:12950917]
[WorldCat.org]
[DOI]
(P p)
Huang-Mo Sung, Ronald E Yasbin
Adaptive, or stationary-phase, mutagenesis, a component of bacterial differentiation in Bacillus subtilis.
J Bacteriol: 2002, 184(20);5641-53
[PubMed:12270822]
[WorldCat.org]
[DOI]
(P p)
Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344]
[WorldCat.org]
[DOI]
(P p)
A Yazgan, G Ozcengiz, M A Marahiel
Tn10 insertional mutations of Bacillus subtilis that block the biosynthesis of bacilysin.
Biochim Biophys Acta: 2001, 1518(1-2);87-94
[PubMed:11267663]
[WorldCat.org]
[DOI]
(P p)
P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937]
[WorldCat.org]
[DOI]
(P p)
M Persuh, K Turgay, I Mandic-Mulec, D Dubnau
The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch.
Mol Microbiol: 1999, 33(4);886-94
[PubMed:10447896]
[WorldCat.org]
[DOI]
(P p)
C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672]
[WorldCat.org]
[DOI]
(P p)
M Perego, J A Hoch
Cell-cell communication regulates the effects of protein aspartate phosphatases on the phosphorelay controlling development in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(4);1549-53
[PubMed:8643670]
[WorldCat.org]
[DOI]
(P p)
M Roggiani, D Dubnau
ComA, a phosphorylated response regulator protein of Bacillus subtilis, binds to the promoter region of srfA.
J Bacteriol: 1993, 175(10);3182-7
[PubMed:8387999]
[WorldCat.org]
[DOI]
(P p)
Y Weinrauch, R Penchev, E Dubnau, I Smith, D Dubnau
A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.
Genes Dev: 1990, 4(5);860-72
[PubMed:2116363]
[WorldCat.org]
[DOI]
(P p)
Y Weinrauch, N Guillen, D A Dubnau
Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants.
J Bacteriol: 1989, 171(10);5362-75
[PubMed:2507523]
[WorldCat.org]
[DOI]
(P p)
