Difference between revisions of "PutB"
| Line 4: | Line 4: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''putB'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ycgM'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
| Line 20: | Line 20: | ||
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 909 bp, 303 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 909 bp, 303 aa | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgL]]'', ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgL]]'', ''[[putC]]'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12114]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12114]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | ||
| Line 38: | Line 38: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[CodY regulon]]}}, | {{SubtiWiki regulon|[[CodY regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[PutR regulon]]}}, | ||
{{SubtiWiki regulon|[[Spo0A regulon]]}} | {{SubtiWiki regulon|[[Spo0A regulon]]}} | ||
| Line 47: | Line 48: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * no growth with proline as single source of carbon or nitrogen {{PubMed|21840319}} | ||
=== Database entries === | === Database entries === | ||
| Line 98: | Line 100: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[ | + | * '''Operon:''' ''[[putB]]-[[putC]]-[[putP]]'' {{PubMed|21840319}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21840319}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced in the presence of proline ([[PutR]]) {{PubMed|21840319}} | ||
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | ||
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[PutR]]: transcription activation {{PubMed|21840319}} | ||
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | ||
| − | ** [[CodY]]: transcription repression [ | + | ** [[CodY]]: transcription repression, displacement of [[PutR]] {{PubMed|21840319}} |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 133: | Line 137: | ||
=References= | =References= | ||
| − | <pubmed>14976255,,14651647,12618455, </pubmed> | + | <pubmed>14976255,21840319,14651647,12618455, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:51, 17 August 2011
- Description: proline oxidase (L-proline, NAD)
| Gene name | putB |
| Synonyms | ycgM |
| Essential | no |
| Product | proline oxidase (L-proline, NAD) |
| Function | proline utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 34 kDa, 7.291 |
| Gene length, protein length | 909 bp, 303 aa |
| Immediate neighbours | ycgL, putC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
CodY regulon, PutR regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU03200
Phenotypes of a mutant
- no growth with proline as single source of carbon or nitrogen PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)
- Protein family: proline dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s): FadM
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P94390
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
