UreB

• Description: urease (beta subunit)
  
  

Gene name	ureB
Synonyms
Essential	no
Product	urease (beta subunit)
Function	utilization of urea as alternative nitrogen source
Interactions involving this protein in SubtInteract: UreB
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Ammonium/ glutamate, Alternative nitrogen sources
MW, pI	13 kDa, 9.751
Gene length, protein length	372 bp, 124 aa
Immediate neighbours	ureC, ureA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

CodY regulon, GlnR regulon, PucR regulon, SigH regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU36650

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Urea + H₂O = CO₂ + 2 NH₃ (according to Swiss-Prot)

• Protein family: urease beta subunit family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • UreA-UreB-UreC

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P71035

• KEGG entry: [3]

• E.C. number: 3.5.1.5

Expression and regulation

• Operon: ureA-ureB-ureC PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • induced by nitrogen limitation (GlnR, TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • GlnR: transcription repression PubMed
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, S H Fisher
Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H.
J Bacteriol: 1997, 179(17);5494-501
[PubMed:9287005] [WorldCat.org] [DOI] (P p)