UreA

• Description: urease (gamma subunit)
  
  

Gene name	ureA
Synonyms
Essential	no
Product	urease (gamma subunit)
Function	utilization of urea as alternative nitrogen source
Interactions involving this protein in SubtInteract: UreA
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Ammonium/ glutamate, Alternative nitrogen sources
MW, pI	11 kDa, 4.98
Gene length, protein length	315 bp, 105 aa
Immediate neighbours	ureB, csbD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

CodY regulon, GlnR regulon, PucR regulon, SigH regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU36660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Urea + H₂O = CO₂ + 2 NH₃ (according to Swiss-Prot)

• Protein family: urease gamma subunit family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • UreA-UreB-UreC

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2FVH (from Mycobacterium tuberculosis, 57% identity, 62% similarity)

• UniProt: P75030

• KEGG entry: [3]

• E.C. number: 3.5.1.5

Expression and regulation

• Operon: ureA-ureB-ureC PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • induced by nitrogen limitation (GlnR, TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • GlnR: transcription repression PubMed
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: