SigB

• Description: RNA polymerase sigma factor SigB
  
  

Gene name	sigB
Synonyms	rpoF
Essential	no
Product	RNA polymerase sigma factor SigB
Function	general stress response
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	29 kDa, 5.418
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	rsbW, rsbX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

transcription, sigma factors and their control, general stress proteins (controlled by SigB)

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SigB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Genes/ operons controlled by SigB

csbB, ctc, gsiB, katE, lexA,licR, sfA, xpf, yaaH, ydeC, ytzE, yvaN, ywhH,

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SigB-RsbW PubMed, SigB-RpoB-RpoC

• Localization:

Database entries

• Structure:

• UniProt: P06574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed
  • rsbV-rsbW-sigB-rsbX PubMed

• Sigma factor:
  • rsbR: SigA PubMed
  • rsbV: SigB PubMed

• Regulation:
  • rsbV: induced by stress (SigB) PubMed

• Additional information:

Biological materials

• Mutant: QB5344 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Reviews

Jon Marles-Wright, Richard J Lewis
The stressosome: molecular architecture of a signalling hub.
Biochem Soc Trans: 2010, 38(4);928-33
[PubMed:20658979] [WorldCat.org] [DOI] (I p)

Peter Zuber
Management of oxidative stress in Bacillus.
Annu Rev Microbiol: 2009, 63;575-97
[PubMed:19575568] [WorldCat.org] [DOI] (I p)

Michael Hecker, Jan Pané-Farré, Uwe Völker
SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria.
Annu Rev Microbiol: 2007, 61;215-36
[PubMed:18035607] [WorldCat.org] [DOI] (P p)

M Hecker, U Völker
General stress response of Bacillus subtilis and other bacteria.
Adv Microb Physiol: 2001, 44;35-91
[PubMed:11407115] [WorldCat.org] [DOI] (P p)

M Hecker, U Völker
Non-specific, general and multiple stress resistance of growth-restricted Bacillus subtilis cells by the expression of the sigmaB regulon.
Mol Microbiol: 1998, 29(5);1129-36
[PubMed:9767581] [WorldCat.org] [DOI] (P p)