SigB

• Description: RNA polymerase sigma factor SigB
  
  

Gene name	sigB
Synonyms	rpoF
Essential	no
Product	RNA polymerase sigma factor SigB
Function	general stress response
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	29 kDa, 5.418
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	rsbW, rsbX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SigB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Genes/ operons controlled by SigB

csbB, ctc, gsiB, katE, lexA,licR, sfA, xpf, yaaH, ydeC, ytzE, yvaN, ywhH,

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SigB-RsbW PubMed, SigB-RpoB-RpoC

• Localization:

Database entries

• Structure:

• UniProt: P06574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed
  • rsbV-rsbW-sigB-rsbX PubMed

• Sigma factor:
  • rsbR: SigA PubMed
  • rsbV: SigB PubMed

• Regulation:
  • rsbV: induced by stress (SigB) PubMed

• Additional information:

Biological materials

• Mutant: QB5344 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Identification of the SigB regulon

J D Helmann, M F Wu, P A Kobel, F J Gamo, M Wilson, M M Morshedi, M Navre, C Paddon
Global transcriptional response of Bacillus subtilis to heat shock.
J Bacteriol: 2001, 183(24);7318-28
[PubMed:11717291] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)