FolK

• Description: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
  
  

Gene name	folK
Synonyms
Essential	no
Product	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
Function	biosynthesis of folate
Metabolic function and regulation of this protein in SubtiPathways: Folate
MW, pI	18 kDa, 4.531
Gene length, protein length	501 bp, 167 aa
Immediate neighbours	folB, yazB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00790

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate (according to Swiss-Prot)

• Protein family: HPPK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1IM6 (from Escherichia coli, 47% identity, 61% similarity) PubMed

• UniProt: P29252

• KEGG entry: [3]

• E.C. number: 2.7.6.3

Expression and regulation

• Operon: pabB-pabA-pabC-sul-folB-folK-yazB-yacF-lysS PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Antoine de Saizieu, Pierre Vankan, Cassandra Vockler, Adolphus P G M van Loon
The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon.
Microbiology (Reading): 1997, 143 ( Pt 3);979-989
[PubMed:9084182] [WorldCat.org] [DOI] (P p)