Difference between revisions of "LevB"
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[sacB]]-[[levB]]-[[yveA]]'' {{PubMed|11739774}} | ||
| − | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2428811}} | |
| − | |||
| − | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induction by sucrose (at high concentration) ([[SacY]]) {{PubMed|3039303}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[SacY]]: binding to a [[RNA switch]] (transcriptional antitermination) {{PubMed|3039303}} | ||
| + | ** [[DegU]]-P: transcription activation {{PubMed|2428811}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 119: | Line 121: | ||
=References= | =References= | ||
| − | + | <pubmed>11739774 2428811 3039303</pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:10, 30 December 2009
- Description: endolevanase
| Gene name | levB |
| Synonyms | yveB |
| Essential | no |
| Product | endolevanase |
| Function | levan degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 58 kDa, 4.993 |
| Gene length, protein length | 1548 bp, 516 aa |
| Immediate neighbours | sacB, yveA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU34460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O07003
- KEGG entry: [3]
- E.C. number: 3.2.1.64
Additional information
Expression and regulation
- Regulatory mechanism:
- SacY: binding to a RNA switch (transcriptional antitermination) PubMed
- DegU-P: transcription activation PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Y Pereira, M F Petit-Glatron, R Chambert
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Microbiology (Reading): 2001, 147(Pt 12);3413-9
[PubMed:11739774]
[WorldCat.org]
[DOI]
(P p)
S Aymerich, M Steinmetz
Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase.
Mol Gen Genet: 1987, 208(1-2);114-20
[PubMed:3039303]
[WorldCat.org]
[DOI]
(P p)
H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811]
[WorldCat.org]
[DOI]
(P p)
