Difference between revisions of "PucF"
(→Expression and regulation) |
|||
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[pucF]]-[[pucG]]'' {{PubMed|12029039}} | ||
| − | + | * '''Sigma factor:''' [[SigA]] {{PubMed|12029039}} | |
| − | |||
| − | * '''Sigma factor:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) | + | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [PucR]]: transcription activation | + | ** [[PucR]]: transcription activation {{PubMed|12029039}} |
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 15:41, 28 December 2009
- Description: allantoate amidohydrolase
| Gene name | pucF |
| Synonyms | yurH |
| Essential | no |
| Product | allantoate amidohydrolase |
| Function | purine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation) | |
| MW, pI | 45 kDa, 5.364 |
| Gene length, protein length | 1236 bp, 412 aa |
| Immediate neighbours | pucG, yurI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU32530
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: peptidase M20 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O32149
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136]
[WorldCat.org]
[DOI]
(P p)
