Difference between revisions of "IolA"
| Line 88: | Line 88: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]'' | + | * '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] |
| − | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] |
| − | * '''Regulation:''' repressed by glucose ([[CcpA]]) | + | * '''Regulation:''' |
| + | ** repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed] | ||
| + | ** induced by inositol ([[IolR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] | ||
| − | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression | + | * '''Regulatory mechanism:''' |
| + | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed] | ||
| + | ** [[IolR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 17:09, 1 June 2009
- Description: methylmalonate-semialdehyde dehydrogenase (acylating)
| Gene name | iolA |
| Synonyms | mmsA, yxdA |
| Essential | no |
| Product | methylmalonate-semialdehyde dehydrogenase (acylating) |
| Function | myo-inositol catabolism |
| MW, pI | 53 kDa, 5.139 |
| Gene length, protein length | 1461 bp, 487 aa |
| Immediate neighbours | iolB, iolR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H (according to Swiss-Prot)
- Protein family: IolA subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1T90
- Swiss prot entry: P42412
- KEGG entry: BSU39760
- E.C. number: 1.2.1.27
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y. (2008) myo-Inositol catabolism in Bacillus subtilis. J Biol Chem. Apr 18;283(16): 10415-24. PubMed
