Difference between revisions of "SacB"
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=== Basic information === | === Basic information === | ||
| − | * ''' | + | * '''Locus tag:''' |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
Revision as of 13:04, 2 June 2009
- Description: levansucrase
| Gene name | sacB |
| Synonyms | |
| Essential | no |
| Product | levansucrase |
| Function | utilization of sucrose, production of levan |
| MW, pI | 52 kDa, 6.175 |
| Gene length, protein length | 1419 bp, 473 aa |
| Immediate neighbours | pbpE, levB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
Database entries
- Swiss prot entry: P05655
- KEGG entry: BSU34450
- E.C. number: 2.4.1.10
Additional information
Expression and regulation
- Operon:
- Regulation: induction by sucrose (at high concentration)
- Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination), in addition activated by DegU-P
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
- Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
