FabF

• Description: beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity
  
  

Gene name	fabF
Synonyms	yjaY
Essential	yes PubMed
Product	beta-ketoacyl-acyl carrier protein synthase II
Function	fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabF
Metabolic function and regulation of this protein in SubtiPathways: fabF
MW, pI	43 kDa, 4.768
Gene length, protein length	1239 bp, 413 aa
Immediate neighbours	fabHA, yjaZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane dynamics, biosynthesis of lipids, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

FapR regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU11340

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU11340

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO₂ + [acyl-carrier-protein] (according to Swiss-Prot)
  • SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed

• Protein family: beta-ketoacyl-ACP synthases family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU11340

• Structure: 4LS5 PubMed

• UniProt: O34340

• KEGG entry: [3]

• E.C. number: 2.3.1.179

Expression and regulation

• Operon: fabHA-fabF PubMed

• Expression browser: fabF PubMed

• Sigma factor:
  • fabHA: SigA PubMed
  • fabF: SigW PubMed

• Regulation:
  • fabHA: expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • fabHA: induced upon fatty acid biosynthesis inhibition PubMed
  • fabF: induced by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100 (SigW) PubMed

• Regulatory mechanism:
  • FapR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)