AnsZ

• Description: asparaginase
  
  

Gene name	ansZ
Synonyms	yccC
Essential	no
Product	asparaginase
Function	asparagine utilization
Gene expression levels in SubtiExpress: ansZ
Metabolic function and regulation of this protein in SubtiPathways: ansZ
MW, pI	39 kDa, 6.15
Gene length, protein length	1125 bp, 375 aa
Immediate neighbours	lmrA, lip
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU02690

Database entries

• BsubCyc: BSU02690

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

• Protein family: asparaginase 1 family (according to Swiss-Prot)

• Paralogous protein(s): AnsA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU02690

• Structure: 1ZCF (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)

• UniProt: O34482

• KEGG entry: [3]

• E.C. number: 3.5.1.1

Expression and regulation

• Operon: ansZ PubMed

• Expression browser: ansZ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mingmei Jia, Meijuan Xu, Beibei He, Zhiming Rao
Cloning, expression, and characterization of L-asparaginase from a newly isolated Bacillus subtilis B11-06.
J Agric Food Chem: 2013, 61(39);9428-34
[PubMed:24003863] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)