Difference between revisions of "SecA"

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* '''Description:''' preprotein translocase subunit (ATPase) <br/><br/>
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* '''Description:''' preprotein translocase subunit (ATPase), required for membrane targeting of [[DivIVA]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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=== Additional information===
 
=== Additional information===
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−
 
−
 
  
 
=The protein=
 
=The protein=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' ATP -> ADP + Pi + preprotein translocation
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* '''Catalyzed reaction/ biological activity:'''  
 +
** ATP -> ADP + Pi + preprotein translocation
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** required for membrane targeting of [[DivIVA]] {{PubMed|24592260}}
  
 
* '''Protein family:''' SecA family (according to Swiss-Prot)
 
* '''Protein family:''' SecA family (according to Swiss-Prot)
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:''' nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
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* '''[[Domains]]:''' nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
  
 
* '''Modification:'''
 
* '''Modification:'''
  
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* '''Cofactor(s):''' magnesium
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* '''[[Cofactors]]:''' Mg
  
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* '''Effectors of protein activity:''' anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) [http://www.ncbi.nlm.nih.gov/pubmed/19924216 PubMed]
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* '''Effectors of protein activity:''' anionic phospholipids, preprotein, [[SecY]], signal peptides (even when added in trans) [http://www.ncbi.nlm.nih.gov/pubmed/19924216 PubMed]
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 
** ([[SecY]]-[[SecE]]-[[SecG]])2-[[SecA]]2 {{PubMed|18923516}}
 
** ([[SecY]]-[[SecE]]-[[SecG]])2-[[SecA]]2 {{PubMed|18923516}}
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** [[SecA]]-[[Ffh]] {{PubMed|9880811}}, [[CsaA]]-[[SecA]] {{PubMed|10816431}}
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** [[SecA]]-[[Ffh]] {{PubMed|9880811}}
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** [[CsaA]]-[[SecA]] {{PubMed|10816431}}
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** [[DivIVA]]-[[SecA]] {{PubMed|24592260}}
  
 
* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
 
* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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=== Additional information===
 
=== Additional information===
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+
* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
 
  
 
=Expression and regulation=
 
=Expression and regulation=
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=References=
 
=References=
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<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 </pubmed>
+
<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:48, 5 March 2014

  • Description: preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA

Gene name secA
Synonyms div, div-341, ts-341
Essential yes PubMed
Product preprotein translocase subunit (ATPase)
Function protein secretion
Gene expression levels in SubtiExpress: secA
Interactions involving this protein in SubtInteract: SecA
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 95 kDa, 5.34
Gene length, protein length 2523 bp, 841 aa
Immediate neighbours prfB, yvyD
Sequences Protein DNA DNA_with_flanks
Genetic context
SecA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SecA expression.png















Categories containing this gene/protein

protein secretion, essential genes, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU35300

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP -> ADP + Pi + preprotein translocation
    • required for membrane targeting of DivIVA PubMed
  • Protein family: SecA family (according to Swiss-Prot)
  • Paralogous protein(s): none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range

Extended information on the protein

  • Kinetic information:
  • Domains: nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
  • Modification:
  • Effectors of protein activity: anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

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