prfB
168

peptide chain release factor 2, recognize stop codon and hydrolyze the completed protein, RF2 recognizes UAA and UGA

Locus

BSU_35290

Molecular weight

41.91 kDa

Isoelectric point

4.85

Protein length

366 aaSequenceBlast

Gene length

1102 bpSequenceBlast

Function

translation

Product

peptide chain release factor 2

Essential

yes

Synonyms

prfB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1186 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,627,139 3,628,240

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA PubMed

Protein family

prokaryotic/mitochondrial release factor family (with PrfA, according to UniProt)

Structure

1MI6 (PDB) (from E. coli, 45% identity) PubMed

P28367 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes

secA-prfB

Description