HutH

• Description: histidase
  
  

Gene name	hutH
Synonyms
Essential	no
Product	histidase
Function	histidine utilization
Gene expression levels in SubtiExpress: hutH
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	55 kDa, 5.221
Gene length, protein length	1524 bp, 508 aa
Immediate neighbours	hutP, hutU
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, HutP regulon

The gene

Basic information

• Locus tag: BSU39350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-histidine = urocanate + NH₃ (according to Swiss-Prot)

• Protein family: PAL/histidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1B8F (from Pseudomonas putida, 43% identity, 62% similarity) PubMed

• UniProt: P10944

• KEGG entry: [3]

• E.C. number: 4.3.1.3

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Expression browser: hutH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by histidine (HutP) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • CodY: transcription repression PubMed
  • HutP: transcriptional antitermination at a protein-dependent RNA switch located between hutP and hutH PubMed

• Additional information:

Biological materials

• Mutant: GP1114 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Robert A Bender
Regulation of the histidine utilization (hut) system in bacteria.
Microbiol Mol Biol Rev: 2012, 76(3);565-84
[PubMed:22933560] [WorldCat.org] [DOI] (I p)