LevB

• Description: endolevanase
  
  

Gene name	levB
Synonyms	yveB
Essential	no
Product	endolevanase
Function	levan degradation
Gene expression levels in SubtiExpress: levB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	58 kDa, 4.993
Gene length, protein length	1548 bp, 516 aa
Immediate neighbours	sacB, yveA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

• Locus tag: BSU34460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07003

• KEGG entry: [3]

• E.C. number: 3.2.1.64

Expression and regulation

• Operon: sacB-levB-yveA PubMed

• Expression browser: levB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by sucrose (at high concentration) (SacY) PubMed

• Regulatory mechanism:
  • SacY: binding to a RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: