FadE

• Description: acyl-CoA dehydrogenase
  
  

Gene name	fadE
Synonyms	yusJ
Essential	no
Product	acyl-CoA dehydrogenase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadE
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	65 kDa, 5.146
Gene length, protein length	1782 bp, 594 aa
Immediate neighbours	yusI, fadA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

utilization of lipids, membrane proteins

This gene is a member of the following regulons

CcpA regulon, FadR regulon, SdpR regulon

The gene

Basic information

• Locus tag: BSU32820

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF (according to Swiss-Prot)

• Protein family: acyl-CoA dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YngJ, MmgC, AcdA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane PubMed

Database entries

• Structure: 2Z1Q (from Thermus thermophilus hb8, 52% identity, 68% similarity)

• UniProt: O32176

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • fadM-fadN-fadA-fadE PubMed
  • fadN-fadA-fadE PubMed

• Sigma factor:
  • fadN: SigA PubMed

• Regulation:
  • fadN: induced by long-chain fatty acids (FadR) PubMed
  • fadN: subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • SdpR: transcription activation PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086] [WorldCat.org] [DOI] (I p)