MtnK

• Description: 5-methylthioribose kinase
  
  

Gene name	mtnK
Synonyms	ykrT
Essential	no
Product	5-methylthioribose kinase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnK
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	45 kDa, 4.856
Gene length, protein length	1197 bp, 399 aa
Immediate neighbours	mtnA, mtnU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins, phosphoproteins

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate (according to Swiss-Prot)

• Protein family: methylthioribose kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-80 OR Arg-82 and Arg-365 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• Structure: 2PUP

• UniProt: O31663

• KEGG entry: [3]

• E.C. number: 2.7.1.100

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: mtnK-mtnA PubMed

• Expression browser: mtnK PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: