Difference between revisions of "Fur"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
* no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to [[FsrA]]-mediated repression of the ''[[gltA]]-[[gltB]]'' operon) {{PubMed|22389480}} | * no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to [[FsrA]]-mediated repression of the ''[[gltA]]-[[gltB]]'' operon) {{PubMed|22389480}} | ||
| + | * poor frowth on lactate as single carbon source (due to overexpression of [[FsrA]]-mediated repression of the ''[[lutA]]-[[lutB]]-[[lutC]]'' operon, can be suppressed by inactivation of ''[[fsrA]]'' or ''[[fbpB]]'') {{PubMed|22427629}} | ||
* transcription profile of a ''[[fur]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27416 GEO] {{PubMed|22389480}} | * transcription profile of a ''[[fur]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27416 GEO] {{PubMed|22389480}} | ||
| Line 142: | Line 143: | ||
<pubmed> 12374814,12354229, 22389480 </pubmed> | <pubmed> 12374814,12354229, 22389480 </pubmed> | ||
==Other original publications== | ==Other original publications== | ||
| − | <pubmed>18487332,14563870,18697947,10400588,11790741,16672620,12207695,9701813,12029044,,17725565,17012385,12950915,19508286 , 16672620, </pubmed> | + | <pubmed>18487332,14563870,18697947,10400588,11790741,16672620,12207695,9701813,12029044,22427629,17725565,17012385,12950915,19508286 , 16672620, </pubmed> |
[http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:15, 25 March 2012
- Description: transcription regulator of iron homoeostasis
| Gene name | fur |
| Synonyms | yqkL |
| Essential | no |
| Product | transcriptional repressor |
| Function | regulation of iron homoeostasis
and transcription of ferri-siderophore uptake genes |
| Metabolic function and regulation of this protein in SubtiPathways: Metal ion homeostasis, Alternative nitrogen sources, Protein secretion | |
| MW, pI | 17 kDa, 5.374 |
| Gene length, protein length | 447 bp, 149 aa |
| Immediate neighbours | ripX, spoIIM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
acquisition of iron, iron metabolism, transcription factors and their control
This gene is a member of the following regulons
The Fur regulon
The gene
Basic information
- Locus tag: BSU23520
Phenotypes of a mutant
- no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to FsrA-mediated repression of the gltA-gltB operon) PubMed
- poor frowth on lactate as single carbon source (due to overexpression of FsrA-mediated repression of the lutA-lutB-lutC operon, can be suppressed by inactivation of fsrA or fbpB) PubMed
- transcription profile of a fur mutant strain: GEO PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: Fur family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): contains an iron-sulfur cluster
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P54574
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: fur PubMed
- Additional information:
Biological materials
- Mutant: HB6543 (aphA3), available in the John Helmann lab; also available in the Stülke lab GP879 (fur::mls) and GP868 (fur::mls, perR::spc)
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
John Helmann, Cornell University, USA Homepage
Your additional remarks
References
Reviews
Charles M Moore, John D Helmann
Metal ion homeostasis in Bacillus subtilis.
Curr Opin Microbiol: 2005, 8(2);188-95
[PubMed:15802251]
[WorldCat.org]
[DOI]
(P p)
The Fur regulon
Addititonal publications: PubMed
Other original publications
