Difference between revisions of "ComEA"
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| − | * '''Description:''' membrane DNA receptor <br/><br/> | + | * '''Description:''' membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake <br/><br/> |
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * strongly reduced transformation rate {{PubMed|22398145}} | ||
=== Database entries === | === Database entries === | ||
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<pubmed> 15083159 </pubmed> | <pubmed> 15083159 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>9987128,11814663,7768800,16751195,7968523,17630974, 21278288,19028902, 21707789 </pubmed> | + | <pubmed>9987128,11814663,7768800,16751195,7968523,17630974, 21278288,19028902, 21707789 22398145</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 18:18, 14 March 2012
- Description: membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake
| Gene name | comEA |
| Synonyms | comD |
| Essential | no |
| Product | membrane DNA receptor |
| Function | genetic competence |
| Interactions involving this protein in SubtInteract: ComEA | |
| Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Protein secretion | |
| MW, pI | 21 kDa, 4.824 |
| Gene length, protein length | 615 bp, 205 aa |
| Immediate neighbours | comEB, comER |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
genetic competence, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25590
Phenotypes of a mutant
- strongly reduced transformation rate PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane, no distinct position PubMed
Database entries
- Structure: 2DUY (from Thermus thermophilus hb8, 37% identity, 60% similarity)
- UniProt: P39694
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159]
[WorldCat.org]
[DOI]
(P p)
Original publications
Masaomi Takeno, Hisataka Taguchi, Takashi Akamatsu
Role of ComEA in DNA uptake during transformation of competent Bacillus subtilis.
J Biosci Bioeng: 2012, 113(6);689-93
[PubMed:22398145]
[WorldCat.org]
[DOI]
(I p)
Kenneth Briley, Angella Dorsey-Oresto, Peter Prepiak, Miguel J Dias, Jessica M Mann, David Dubnau
The secretion ATPase ComGA is required for the binding and transport of transforming DNA.
Mol Microbiol: 2011, 81(3);818-30
[PubMed:21707789]
[WorldCat.org]
[DOI]
(I p)
Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J Bacteriol: 2011, 193(7);1633-42
[PubMed:21278288]
[WorldCat.org]
[DOI]
(I p)
Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902]
[WorldCat.org]
[DOI]
(I p)
Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974]
[WorldCat.org]
[DOI]
(P p)
Inês Chen, Roberta Provvedi, David Dubnau
A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis.
J Biol Chem: 2006, 281(31);21720-21727
[PubMed:16751195]
[WorldCat.org]
[DOI]
(P p)
Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663]
[WorldCat.org]
[DOI]
(P p)
R Provvedi, D Dubnau
ComEA is a DNA receptor for transformation of competent Bacillus subtilis.
Mol Microbiol: 1999, 31(1);271-80
[PubMed:9987128]
[WorldCat.org]
[DOI]
(P p)
G S Inamine, D Dubnau
ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport.
J Bacteriol: 1995, 177(11);3045-51
[PubMed:7768800]
[WorldCat.org]
[DOI]
(P p)
J Hahn, G Inamine, Y Kozlov, D Dubnau
Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.
Mol Microbiol: 1993, 10(1);99-111
[PubMed:7968523]
[WorldCat.org]
[DOI]
(P p)
