Crh

• Description: "Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor
  
  

Gene name	crh
Synonyms	yvcM
Essential	no
Product	carbon-flux regulating HPr
Function	control of carbon flux
Interactions involving this protein in SubtInteract: Crh
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	9,2 kDa, 4.70
Gene length, protein length	255 bp, 85 amino acids
Immediate neighbours	yvcN, yvcL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription factors and their control, phosphoproteins

The gene

Basic information

• Locus tag: BSU34740

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: HPr family

• Paralogous protein(s): HPr

Extended information on the protein

• Kinetic information:

• Domains: HPr domain (1–85)

• Modification: phosphorylation on Ser46 by HprK PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Crh-GapA PubMed
  • Crh-CcpA PubMed
  • HprK-Crh PubMed
  • Crh-MgsA (only non-phosphorylated Crh interacts with MgsA) PubMed

• Localization:

Database entries

• Structure:
  • 2AK7 (dimeric Crh-Ser46-P)
  • 1ZVV (CcpA-Crh-DNA complex)
  • 2RLZ (dimer)
  • 1MU4
  • NCBI, dimer
  • NCBI, CcpA-Crh-DNA complex
  • NCBI, dimeric phosphor-Crh
  • NCBI
• UniProt: O06976

• KEGG entry: [2]

Additional information

Crh does not possess the phosphorylation site used for PTS phosphotransfer (His-15 in PtsH), it can only be phosphorylated on Ser-46

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information: Crh is weakly expressed. This results in part from a poorly conserved ribosomal binding site of the mRNA. PubMed

Biological materials

• Mutant: GP860 (aphA3), QB7097 (spc), available in Stülke lab

• Expression vector:
  • pGP641 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • pGP734 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab

• lacZ fusion: see yvcI

• GFP fusion:

• two-hybrid system: crh, crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• FLAG-tag construct:
  • GP69 (spc, based on pGP1331), available in the Boris Görke and Stülke labs
  • pGP1200 crh(Ser46Ala) (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Wolfgang Hillen, Erlangen University, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage