BkdAA

• Description: 2-oxoisovalerate dehydrogenase (E1 alpha subunit)
  
  

Gene name	bkdAA
Synonyms	bfmBAA, bfmB1a, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E1 alpha subunit)
Function	utilization of branched-chain keto acids
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val
MW, pI	36 kDa, 4.778
Gene length, protein length	990 bp, 330 aa
Immediate neighbours	bkdAB, lpdV
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family: BCKDHA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• Structure: 1UMB (from Thermus thermophilus, 42% identity, 57% similarity) PubMed

• UniProt: P37940

• KEGG entry: [3]

• E.C. number: 1.2.4.4

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced in the presence of isoleucine or valine (BkdR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • BkdR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: