SacB

• Description: levansucrase
  
  

Gene name	sacB
Synonyms
Essential	no
Product	levansucrase
Function	utilization of sucrose, production of levan
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress
MW, pI	52 kDa, 6.175
Gene length, protein length	1419 bp, 473 aa
Immediate neighbours	pbpE, levB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure: 1PT2 (complex with sucrose), 1OYG

• UniProt: P05655

• KEGG entry: [3]

• E.C. number: 2.4.1.10

Expression and regulation

• Operon: sacB-levB-yveA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by sucrose (at high concentration) (SacY) PubMed

• Regulatory mechanism:
  • SacY: binding to a RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)