Difference between revisions of "Sandbox"

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* '''Description:''' phospho-alpha-glucosidase <br/><br/>
+
* '''Description:''' trehalose-specific phosphotransferase system, EIIBC component <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''treA''
+
|''treP''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''treC ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''treB ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-alpha-glucosidase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || trehalose-specific phosphotransferase system, EIIBC component
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || trehalose utilization
+
|style="background:#ABCDEF;" align="center"|'''Function''' || trehalose uptake and phosphorylation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 4.895  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 8.555  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1683 bp, 561 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1410 bp, 470 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[treP]]'', ''[[treR]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfkQ]]'', ''[[treA]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:treA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:treP_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trePAR.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11010]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11009]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:'''
+
* '''Protein family:''' PTS permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07810]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07800]
  
* '''E.C. number:''' 3.2.1.93
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP1118 (spc), available in the [[Stülke]] lab
+
* '''Mutant:'''
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
Line 118: Line 118:
 
=References=
 
=References=
  
 +
# Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''. ''Microbiology'' '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
  
# Helfert C, Gotsche S, Dahl MK. (1995) Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene. ''Mol Microbiol. '' '''Apr;16(1):''' 111-120. [http://www.ncbi.nlm.nih.gov/sites/entrez/7651129 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 20:50, 15 April 2009

  • Description: trehalose-specific phosphotransferase system, EIIBC component

Gene name treP
Synonyms treB
Essential no
Product trehalose-specific phosphotransferase system, EIIBC component
Function trehalose uptake and phosphorylation
MW, pI 49 kDa, 8.555
Gene length, protein length 1410 bp, 470 aa
Immediate neighbours yfkQ, treA
Hier soll was neues rein
Genetic context
TreP context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed