Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' 2-oxoisovalerate dehydrogenase (E1 beta subunit) <br/><br/>
+
* '''Description:''' 2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bkdAB''
+
|''bkdB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''bfmBAB, bfmB1b, bkd ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''bfmBB, bfmB2, bkd ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || 2-oxoisovalerate dehydrogenase (E1 beta subunit)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || 2-oxoisovalerate dehydrogenase <br/>(E2 subunit, lipoamide acyltransferase)  
 
 
(2-oxoisovalerate dehydrogenase beta subunit) (EC 1.2.4.4)
 
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
 
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 4.791  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 5.301  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 981 bp, 327 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1272 bp, 424 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bkdB]]'', ''[[bkdAA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bmrR]]'', ''[[bkdAB]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:bkdAB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:bkdB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 31: Line 29:
 
__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 45: Line 43:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10306]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10305]
  
 
=== Additional information===
 
=== Additional information===
Line 74: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
* '''Localization:''' Nucleoid (Heterogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 82: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24040]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24030]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 18:51, 15 April 2009

  • Description: 2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)

Gene name bkdB
Synonyms bfmBB, bfmB2, bkd
Essential no
Product 2-oxoisovalerate dehydrogenase
(E2 subunit, lipoamide acyltransferase)
Function utilization of branched-chain keto acids
MW, pI 45 kDa, 5.301
Gene length, protein length 1272 bp, 424 aa
Immediate neighbours bmrR, bkdAB
Hier soll was neues rein
Genetic context
BkdB context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Nucleoid (Heterogeneous) PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed