Difference between revisions of "Sandbox"

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* '''Description:''' transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon <br/><br/>
+
* '''Description:''' valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenas <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bkdR''
+
|''bcd''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiR ''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''bkd, yqiT ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator (for SigL-dependent promoter))
+
|style="background:#ABCDEF;" align="center"| '''Product''' || valine dehydrogenase, isoleucine dehydrogenase, <br/>L-leucine dehydrogenas
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of branched-chain amino acid utilization, required for survival at low temperatures)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 77 kDa, 6.237  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.942  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2076 bp, 692 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1092 bp, 364 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptb]]'', ''[[yqzF]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[buk]]'', ''[[ptb]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:bkdR_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:bcd_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/>
  
 
=The gene=
 
=The gene=
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
The mutant is cold-sensitive [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774  PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bkdR.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11721]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11723]
  
 
=== Additional information===
 
=== Additional information===
Line 82: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24100]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24080]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]''
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 118:
 
=References=
 
=References=
  
# Wiegeshoff et al.  (2006) Sigma L is important for cold shock adaptation of ''Bacillus subtilis''. J Bacteriol , 188: 3130-3133. [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774  PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 18:51, 15 April 2009

  • Description: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenas

Gene name bcd
Synonyms bkd, yqiT
Essential no
Product valine dehydrogenase, isoleucine dehydrogenase,
L-leucine dehydrogenas
Function utilization of branched-chain keto acids
MW, pI 39 kDa, 4.942
Gene length, protein length 1092 bp, 364 aa
Immediate neighbours buk, ptb
Hier soll was neues rein
Genetic context
Bcd context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed