ComA

• Description: two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)
  
  

Gene name	comA
Synonyms	srfB, comAA
Essential	no
Product	two-component system response regulator
Function	regulation of genetic competence and quorum sensing
Gene expression levels in SubtiExpress: comA
Interactions involving this protein in SubtInteract: ComA
MW, pI	23 kDa, 4.759
Gene length, protein length	642 bp, 214 aa
Immediate neighbours	yuxO, comP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, phosphoproteins

The gene

Basic information

• Locus tag: BSU31680

Database entries

• BsubCyc: BSU31680

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ComA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by ComP on an Asp residue

• Cofactors:

• Effectors of protein activity:
  • phosphorylation activates DNA binding
  • DNA-binding activity of ComA is inhibited by RapC PubMed, RapD PubMed, RapF PubMed, RapH PubMed and RapK PubMed

• Interactions:
  • RapC-ComA PubMed
  • RapF-ComA PubMed
  • RapH-ComA PubMed
  • ComP-ComA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU31680

• Structure:
  • 1U83
  • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

• UniProt: P14204

• KEGG entry: [3]

• E.C. number: 4.4.1.19

Expression and regulation

• Operon: comA-yuxO PubMed

• Expression browser: comA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 320 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 623 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Daniel López, Roberto Kolter
Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis.
FEMS Microbiol Rev: 2010, 34(2);134-49
[PubMed:20030732] [WorldCat.org] [DOI] (I p)

Leendert W Hamoen, Gerard Venema, Oscar P Kuipers
Controlling competence in Bacillus subtilis: shared use of regulators.
Microbiology (Reading): 2003, 149(Pt 1);9-17
[PubMed:12576575] [WorldCat.org] [DOI] (P p)