PurA

• Description: adenylosuccinate synthetase
  
  

Gene name	purA
Synonyms
Essential	no
Product	adenylosuccinate synthetase
Function	purine biosynthesis
Gene expression levels in SubtiExpress: purA
Metabolic function and regulation of this protein in SubtiPathways: purA
MW, pI	47 kDa, 5.459
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	trnY-Lys, yycE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

• Locus tag: BSU40420

Database entries

• BsubCyc: BSU40420

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP (according to Swiss-Prot)

• Protein family: adenylosuccinate synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-97 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU40420

• Structure: 1KJX (from Escherichia coli k12, 46% identity, 64% similarity) PubMed

• UniProt: P29726

• KEGG entry: [3]

• E.C. number: 6.3.4.4

Expression and regulation

• Operon: purA PubMed

• Expression browser: purA PubMed

• Sigma factor: SigA PubMed

• Regulation: expression activated by glucose (4.9 fold) (CcpA) PubMed
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism:
  • PurR: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1965 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 9672 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: