CcpC

• Description: transcriptional repressor of citZ and citB
  
  

Gene name	ccpC
Synonyms	ykuM
Essential	no
Product	transcriptional repressor (LysR family)
Function	regulation of tricarboxylic acid branch of the TCA cycle
Gene expression levels in SubtiExpress: ccpC
Metabolic function and regulation of this protein in SubtiPathways: ccpC
MW, pI	33 kDa, 5.932
Gene length, protein length	879 bp, 293 aa
Immediate neighbours	ykuL, ykuN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

CcpA regulon, CcpC regulon

The CcpC regulon:

• ccpC, citB, citZ-icd-mdh

The gene

Basic information

• Locus tag: BSU14140

Database entries

• BsubCyc: BSU14140

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LysR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU14140

• Structure:

• UniProt: O34827

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ykuJ-ykuK-abbA-ykuL-ccpC PubMed
  • ccpC PubMed

• Expression browser: ccpC PubMed

• Sigma factor:
  • ccpC: SigA

• Regulation:
  • repressed in the presence of glucose and glutamate (CcpC) PubMed

• Regulatory mechanism:
  • CcpC: transcription repression (molecular inducer: citrate) PubMed
  • CcpA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 79 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 101 PubMed

Biological materials

• Mutant: GP206 (aphA3) & GP1442 (aphA3) , available in Stülke lab

• Expression vector:
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP706, available in Stülke lab

• lacZ fusion: pGP707 (in pAC5), available in Stülke lab, pGP711 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

Meghna Mittal, Kieran B Pechter, Silvia Picossi, Hyun-Jin Kim, Kathryn O Kerstein, Abraham L Sonenshein
Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis.
Microbiology (Reading): 2013, 159(Pt 1);68-76
[PubMed:23139400] [WorldCat.org] [DOI] (I p)

Christina Herzberg, Lope Andrés Flórez Weidinger, Bastian Dörrbecker, Sebastian Hübner, Jörg Stülke, Fabian M Commichau
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo.
Proteomics: 2007, 7(22);4032-5
[PubMed:17994626] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Irene Reif, Fabian M Commichau, Christian Detsch, Ingrid Wacker, Holger Ludwig, Jörg Stülke
Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system.
Arch Microbiol: 2006, 185(2);136-46
[PubMed:16395550] [WorldCat.org] [DOI] (P p)

Sam-In Kim, Cécile Jourlin-Castelli, Stephen R Wellington, Abraham L Sonenshein
Mechanism of repression by Bacillus subtilis CcpC, a LysR family regulator.
J Mol Biol: 2003, 334(4);609-24
[PubMed:14636591] [WorldCat.org] [DOI] (P p)

Hyun-Jin Kim, Sam-In Kim, Manoja Ratnayake-Lecamwasam, Kiyoshi Tachikawa, Abraham L Sonenshein, Mark Strauch
Complex regulation of the Bacillus subtilis aconitase gene.
J Bacteriol: 2003, 185(5);1672-80
[PubMed:12591885] [WorldCat.org] [DOI] (P p)

Hyun-Jin Kim, Cécile Jourlin-Castelli, Sam-In Kim, Abraham L Sonenshein
Regulation of the bacillus subtilis ccpC gene by ccpA and ccpC.
Mol Microbiol: 2002, 43(2);399-410
[PubMed:11985717] [WorldCat.org] [DOI] (P p)

C Jourlin-Castelli, N Mani, M M Nakano, A L Sonenshein
CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilis.
J Mol Biol: 2000, 295(4);865-78
[PubMed:10656796] [WorldCat.org] [DOI] (P p)