Difference between revisions of "LeuB"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}, | {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}, | ||
| − | {{SubtiWiki category|[[phosphoproteins]]}} | + | {{SubtiWiki category|[[phosphoproteins]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | <pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,22517742,15547269,12618455,12107147, 20935095 24163341</pubmed> | + | <pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,22517742,15547269,12618455,12107147, 20935095 24163341 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:44, 5 March 2014
- Description: 3-isopropylmalate dehydrogenase
| Gene name | leuB |
| Synonyms | leuC |
| Essential | no |
| Product | 3-isopropylmalate dehydrogenase |
| Function | biosynthesis of leucine |
| Gene expression levels in SubtiExpress: leuB | |
| Metabolic function and regulation of this protein in SubtiPathways: leuB | |
| MW, pI | 39 kDa, 4.744 |
| Gene length, protein length | 1095 bp, 365 aa |
| Immediate neighbours | leuC, leuA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
CcpA regulon, CodY regulon, T-box, TnrA regulon
The gene
Basic information
- Locus tag: BSU28270
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH (according to Swiss-Prot)
- Protein family: LeuB type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- phosphorylated on Arg-4 PubMed
- Effectors of protein activity:
Database entries
- Structure: 1XAD (Thermus thermophilus)
- UniProt: P05645
- KEGG entry: [3]
- E.C. number: 1.1.1.85
Additional information
Expression and regulation
- Regulation:
- for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
- repressed by casamino acids PubMed
- expression is stimulated in the presence of glucose PubMed
- repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
- repressed during growth in the presence of branched chain amino acids (CodY) PubMed
- Regulatory mechanism:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
