TcyK

• Description: cystine ABC transporter (binding protein)
  
  

Gene name	tcyK
Synonyms	ytmK
Essential	no
Product	cystine ABC transporter (binding protein)
Function	cystine uptake
Gene expression levels in SubtiExpress: tcyK
Interactions involving this protein in SubtInteract: TcyK
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD
MW, pI	30 kDa, 9.009
Gene length, protein length	810 bp, 270 aa
Immediate neighbours	tcyL, tcyJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon, AscR regulon

The gene

Basic information

• Locus tag: BSU29370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TcyN-(TcyL-TcyM)-(TcyJ-TcyK) PubMed

• Localization:
  • attached to the membrane via TcyL-TcyM PubMed

Database entries

• Structure: 2O1M

• UniProt: O34852

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM PubMed

• Expression browser: tcyK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed
  • induced in the presence of S-methyl-cysteine (AscR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • AscR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A948 ( tcyK::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: