sndA

sndA
168

sulphur compound N-deacetylase, required for the conversion of S-methyl-cysteine to cysteine

Locus
BSU_29290
Molecular weight
45.08 kDa
Isoelectric point
5.55
Protein length
416 aaSequenceBlast
Gene length
1251 bpSequenceBlast
Function
utilization of S-methyl-cysteine
Product
sulphur compound N-deacetylase
Essential
no
Synonyms
sndA, hipO, ytnL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1473 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CymR regulon, AscR regulon

Gene
Coordinates
2,999,717  3,000,967
Phenotypes of a mutant
a sndA sndB sndC triple mutant does not grow with N-acetyl cysteine and S-(2-succino)cysteine as the single sources of sulfur PubMed
The protein
Catalyzed reaction/ biological activity
deacetylation of N-acetyl-l-cysteine in a Zn2+- or Co2+-dependent reaction PubMed
Protein family
peptidase M20 family (according to UniProt)
Structure
1YSJ (PDB) (SndB, 48% identity)
O34980 (AlphaFold)
Paralogous protein(s)
SndB, SndC, YkuR
Expression and Regulation
Operons
Genes
snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
Description
PubMed
Regulation
induced in the presence of methionine and taurine PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
AscR: activation, PubMed, in ascR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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snaA→ytnM

2025-03-22 23:22:48

Jstuelk

115

8a3868e633778d2f68bf47d615f22f716e843294

4D6CA410A1DC104A491D3C18248CCD50CC0E983A

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
GP4474 (trpC2 ΔsndA::neo), available in Jörg Stülke's lab
BKE29290 (sndA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGACATATGTTCCGCTCCTT,  downstream forward: _UP4_GATTGATATAGATGGGGTGT
BKK29290 (sndA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGACATATGTTCCGCTCCTT,  downstream forward: _UP4_GATTGATATAGATGGGGTGT
References
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Page visits: 4336

Time of last update: 2025-04-02 06:49:32

Author of last update: Robert.warneke