Difference between revisions of "BacD"
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Revision as of 14:51, 16 May 2013
- Description: alanine-anticapsin ligase
| Gene name | bacD |
| Synonyms | ywfE, ipa-83d |
| Essential | no |
| Product | alanine-anticapsin ligase |
| Function | biosynthesis of the antibiotic bacilysin |
| Gene expression levels in SubtiExpress: bacD | |
| MW, pI | 52 kDa, 4.679 |
| Gene length, protein length | 1416 bp, 472 aa |
| Immediate neighbours | bacE, bacC |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, biosynthesis of antibacterial compounds
This gene is a member of the following regulons
AbrB regulon, CodY regulon, ScoC regulon
The gene
Basic information
- Locus tag: BSU37710
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P39641
- KEGG entry: [2]
- E.C. number: 6.3.2.28
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Yasuhito Shomura, Emi Hinokuchi, Hajime Ikeda, Akihiro Senoo, Yuichi Takahashi, Jun-ichi Saito, Hirofumi Komori, Naoki Shibata, Yoshiyuki Yonetani, Yoshiki Higuchi
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.
Protein Sci: 2012, 21(5);707-16
[PubMed:22407814]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406]
[WorldCat.org]
[DOI]
(I p)
Kuniki Kino, Yoichi Kotanaka, Toshinobu Arai, Makoto Yagasaki
A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin.
Biosci Biotechnol Biochem: 2009, 73(4);901-7
[PubMed:19352016]
[WorldCat.org]
[DOI]
(I p)
Kazuhiko Tabata, Hajime Ikeda, Shin-Ichi Hashimoto
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
J Bacteriol: 2005, 187(15);5195-202
[PubMed:16030213]
[WorldCat.org]
[DOI]
(P p)
Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825]
[WorldCat.org]
[DOI]
(P p)
