Difference between revisions of "RsbU"
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| − | + | * '''Description:''' [[Protein kinases and phosphatases|protein serine phosphatase]], environmental PP2C, dephosphorylates [[RsbV]] <br/><br/> | |
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbU_521019_522026_1 rsbU] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbU_521019_522026_1 rsbU] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed] | + | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed] |
* '''Regulation:''' | * '''Regulation:''' | ||
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<pubmed> 20658979 </pubmed> | <pubmed> 20658979 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| + | '''Additional publications:''' {{PubMed|23407164}} | ||
<pubmed>12867438,9786195,8002610,8682769,9658013,8002609,17303566,15342585,8682789,8808936,8824586,16321960,10632888,7601843,15312768,15342582,12950915,8955331, 12499568, 12950928</pubmed> | <pubmed>12867438,9786195,8002610,8682769,9658013,8002609,17303566,15342585,8682789,8808936,8824586,16321960,10632888,7601843,15312768,15342582,12950915,8955331, 12499568, 12950928</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:37, 15 February 2013
- Description: protein serine phosphatase, environmental PP2C, dephosphorylates RsbV
| Gene name | rsbU |
| Synonyms | |
| Essential | no |
| Product | protein serine phosphatase, environmental PP2C |
| Function | control of SigB activity |
| Gene expression levels in SubtiExpress: rsbU | |
| Interactions involving this protein in SubtInteract: RsbU | |
| Metabolic function and regulation of this protein in SubtiPathways: Stress | |
| MW, pI | 38 kDa, 4.906 |
| Gene length, protein length | 1005 bp, 335 aa |
| Immediate neighbours | rsbT, rsbV |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, sigma factors and their control
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04700
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 2J6Y
- UniProt: P40399
- KEGG entry: [3]
- E.C. number: 3.1.3.3
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Bill Haldenwang, San Antonio, USA
- Chet Price, Davis, USA homepage
- Rick Lewis, Newcastle, UK homepage
Your additional remarks
References
Reviews
Jon Marles-Wright, Richard J Lewis
The stressosome: molecular architecture of a signalling hub.
Biochem Soc Trans: 2010, 38(4);928-33
[PubMed:20658979]
[WorldCat.org]
[DOI]
(I p)
Original publications
Additional publications: PubMed
Steven W Hardwick, Jan Pané-Farré, Olivier Delumeau, Jon Marles-Wright, James W Murray, Michael Hecker, Richard J Lewis
Structural and functional characterization of partner switching regulating the environmental stress response in Bacillus subtilis.
J Biol Chem: 2007, 282(15);11562-72
[PubMed:17303566]
[WorldCat.org]
[DOI]
(P p)
Shuyu Zhang, Adam Reeves, Robyn L Woodbury, W G Haldenwang
Coexpression patterns of sigma(B) regulators in Bacillus subtilis affect sigma(B) inducibility.
J Bacteriol: 2005, 187(24);8520-5
[PubMed:16321960]
[WorldCat.org]
[DOI]
(P p)
Gudrun Holtmann, Matthias Brigulla, Leif Steil, Alexandra Schütz, Karsta Barnekow, Uwe Völker, Erhard Bremer
RsbV-independent induction of the SigB-dependent general stress regulon of Bacillus subtilis during growth at high temperature.
J Bacteriol: 2004, 186(18);6150-8
[PubMed:15342585]
[WorldCat.org]
[DOI]
(P p)
Tae-Jong Kim, Tatiana A Gaidenko, Chester W Price
In vivo phosphorylation of partner switching regulators correlates with stress transmission in the environmental signaling pathway of Bacillus subtilis.
J Bacteriol: 2004, 186(18);6124-32
[PubMed:15342582]
[WorldCat.org]
[DOI]
(P p)
Tae-Jong Kim, Tatiana A Gaidenko, Chester W Price
A multicomponent protein complex mediates environmental stress signaling in Bacillus subtilis.
J Mol Biol: 2004, 341(1);135-50
[PubMed:15312768]
[WorldCat.org]
[DOI]
(P p)
Chien-Cheng Chen, Richard J Lewis, Robin Harris, Michael D Yudkin, Olivier Delumeau
A supramolecular complex in the environmental stress signalling pathway of Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1657-69
[PubMed:12950928]
[WorldCat.org]
[DOI]
(P p)
Emmanuel Guedon, Charles M Moore, Qiang Que, Tao Wang, Rick W Ye, John D Helmann
The global transcriptional response of Bacillus subtilis to manganese involves the MntR, Fur, TnrA and sigmaB regulons.
Mol Microbiol: 2003, 49(6);1477-91
[PubMed:12950915]
[WorldCat.org]
[DOI]
(P p)
Matthias Brigulla, Tamara Hoffmann, Andrea Krisp, Andrea Völker, Erhard Bremer, Uwe Völker
Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to low-temperature adaptation.
J Bacteriol: 2003, 185(15);4305-14
[PubMed:12867438]
[WorldCat.org]
[DOI]
(P p)
Sujit Dutta, Richard J Lewis
Crystallization and preliminary crystallographic analysis of the kinase-recruitment domain of the PP2C-type phosphatase RsbU.
Acta Crystallogr D Biol Crystallogr: 2003, 59(Pt 1);191-3
[PubMed:12499568]
[WorldCat.org]
[DOI]
(P p)
K Vijay, M S Brody, E Fredlund, C W Price
A PP2C phosphatase containing a PAS domain is required to convey signals of energy stress to the sigmaB transcription factor of Bacillus subtilis.
Mol Microbiol: 2000, 35(1);180-8
[PubMed:10632888]
[WorldCat.org]
[DOI]
(P p)
C M Kang, K Vijay, C W Price
Serine kinase activity of a Bacillus subtilis switch protein is required to transduce environmental stress signals but not to activate its target PP2C phosphatase.
Mol Microbiol: 1998, 30(1);189-96
[PubMed:9786195]
[WorldCat.org]
[DOI]
(P p)
N Smirnova, J Scott, U Voelker, W G Haldenwang
Isolation and characterization of Bacillus subtilis sigB operon mutations that suppress the loss of the negative regulator RsbX.
J Bacteriol: 1998, 180(14);3671-80
[PubMed:9658013]
[WorldCat.org]
[DOI]
(P p)
U Voelker, A Voelker, W G Haldenwang
The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.
J Bacteriol: 1996, 178(23);7020-3
[PubMed:8955331]
[WorldCat.org]
[DOI]
(P p)
X Yang, C M Kang, M S Brody, C W Price
Opposing pairs of serine protein kinases and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor.
Genes Dev: 1996, 10(18);2265-75
[PubMed:8824586]
[WorldCat.org]
[DOI]
(P p)
U Voelker, A Voelker, W G Haldenwang
Reactivation of the Bacillus subtilis anti-sigma B antagonist, RsbV, by stress- or starvation-induced phosphatase activities.
J Bacteriol: 1996, 178(18);5456-63
[PubMed:8808936]
[WorldCat.org]
[DOI]
(P p)
C M Kang, M S Brody, S Akbar, X Yang, C W Price
Homologous pairs of regulatory proteins control activity of Bacillus subtilis transcription factor sigma(b) in response to environmental stress.
J Bacteriol: 1996, 178(13);3846-53
[PubMed:8682789]
[WorldCat.org]
[DOI]
(P p)
A Dufour, U Voelker, A Voelker, W G Haldenwang
Relative levels and fractionation properties of Bacillus subtilis σ(B) and its regulators during balanced growth and stress.
J Bacteriol: 1996, 178(13);3701-9 sigma
[PubMed:8682769]
[WorldCat.org]
[DOI]
(P p)
U Voelker, A Voelker, B Maul, M Hecker, A Dufour, W G Haldenwang
Separate mechanisms activate sigma B of Bacillus subtilis in response to environmental and metabolic stresses.
J Bacteriol: 1995, 177(13);3771-80
[PubMed:7601843]
[WorldCat.org]
[DOI]
(P p)
A A Wise, C W Price
Four additional genes in the sigB operon of Bacillus subtilis that control activity of the general stress factor sigma B in response to environmental signals.
J Bacteriol: 1995, 177(1);123-33
[PubMed:8002610]
[WorldCat.org]
[DOI]
(P p)
U Voelker, A Dufour, W G Haldenwang
The Bacillus subtilis rsbU gene product is necessary for RsbX-dependent regulation of sigma B.
J Bacteriol: 1995, 177(1);114-22
[PubMed:8002609]
[WorldCat.org]
[DOI]
(P p)
