SigA

• Description: RNA polymerase major sigma factor SigA
  
  

Gene name	sigA
Synonyms	rpoD, crsA
Essential	yes PubMed
Product	RNA polymerase major sigma factor SigA
Function	transcription
Gene expression levels in SubtiExpress: sigA
Interactions involving this protein in SubtInteract: SigA
MW, pI	42 kDa, 4.634
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	cccA, dnaG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, essential genes, membrane proteins

This gene is a member of the following regulons

SigD regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU25200

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• List of SigA controlled genes

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Spo0A-SigA PubMed
  • SigA-(RpoB-RpoC) PubMed
  • BsrA-SigA-RNA polymerase
  • BsrB-SigA-RNA polymerase

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P06224

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • yqxD-dnaG-sigA PubMed
  • sigA PubMed

• Expression browser: sigA PubMed

• Sigma factor:
  • yqxD: SigA PubMed, SigH PubMed
  • sigA: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for sigA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed