SigB

Gene name	sigB
Synonyms	rpoF
Essential	no
Product	RNA polymerase sigma factor SigB
Function	general stress response
Gene expression levels in SubtiExpress: sigB
Interactions involving this protein in SubtInteract: SigB
Metabolic function and regulation of this protein in SubtiPathways: Stress, Murein recycling
MW, pI	29 kDa, 5.418
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	rsbW, rsbX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

CcpA regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU04730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SigB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigB-RsbW PubMed
  • SigB-RpoB-RpoC

• Localization:

Database entries

• Structure:

• UniProt: P06574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed
  • rsbV-rsbW-sigB-rsbX PubMed

• Expression browser: sigB PubMed

• Sigma factor:
  • rsbR: SigA PubMed
  • rsbV: SigB PubMed

• Regulation:
  • rsbV: induced by stress (SigB) PubMed

• Additional information:

Biological materials

• Mutant: QB5344 (cat), available in the Stülke lab
  • 1A675 ( sigB::cat), PubMed, available at BGSC
  • 1A777 ( sigB::cat), PubMed, available at BGSC
  • 1A780 ( sigB::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Reviews

Jon Marles-Wright, Richard J Lewis
The stressosome: molecular architecture of a signalling hub.
Biochem Soc Trans: 2010, 38(4);928-33
[PubMed:20658979] [WorldCat.org] [DOI] (I p)

Peter Zuber
Management of oxidative stress in Bacillus.
Annu Rev Microbiol: 2009, 63;575-97
[PubMed:19575568] [WorldCat.org] [DOI] (I p)

Michael Hecker, Jan Pané-Farré, Uwe Völker
SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria.
Annu Rev Microbiol: 2007, 61;215-36
[PubMed:18035607] [WorldCat.org] [DOI] (P p)

M Hecker, U Völker
General stress response of Bacillus subtilis and other bacteria.
Adv Microb Physiol: 2001, 44;35-91
[PubMed:11407115] [WorldCat.org] [DOI] (P p)

M Hecker, U Völker
Non-specific, general and multiple stress resistance of growth-restricted Bacillus subtilis cells by the expression of the sigmaB regulon.
Mol Microbiol: 1998, 29(5);1129-36
[PubMed:9767581] [WorldCat.org] [DOI] (P p)

Identification of the SigB regulon

Priyanka Nannapaneni, Falk Hertwig, Maren Depke, Michael Hecker, Ulrike Mäder, Uwe Völker, Leif Steil, Sacha A F T van Hijum
Defining the structure of the general stress regulon of Bacillus subtilis using targeted microarray analysis and random forest classification.
Microbiology (Reading): 2012, 158(Pt 3);696-707
[PubMed:22174379] [WorldCat.org] [DOI] (I p)

J D Helmann, M F Wu, P A Kobel, F J Gamo, M Wilson, M M Morshedi, M Navre, C Paddon
Global transcriptional response of Bacillus subtilis to heat shock.
J Bacteriol: 2001, 183(24);7318-28
[PubMed:11717291] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)

Other publications

Additional publications: PubMed

Locke JC, Young JW, Fontes M, Hernández Jiménez MJ, Elowitz MB  
Stochastic pulse regulation in bacterial stress response. 
Science. 2011 334:366-369. 
PubMed:21979936